CRISPR Cas9 Cleavage Detection Kit is a robust and precise RNA-dependent assay used to identify successful insertions and/or deletions in genomic DNA following CRISPR/Cas9 experiments. CRISPR/Cas9 can introduce insertion or deletion mutations (indels) on either one or both copies of a given...
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CRISPR Cas9 Cleavage Detection Kit is a robust and precise RNA-dependent assay used to identify successful insertions and/or deletions in genomic DNA following CRISPR/Cas9 experiments. CRISPR/Cas9 can introduce insertion or deletion mutations (indels) on either one or both copies of a given mammalian gene. The Kit can easily identify if a given clone is monoallelic (has mutations in one copy), biallelic (both copies mutated) or is unedited (Wild-Type) which is considered a major screening advantage over the standard T7E1 Surveyor Assay. This kit uses PCR to first amplify the sgRNA target site from CRISPR edited cells; this amplicon is subsequently cleaved by a ribonucleoprotein (RNP) complex which generates a unique pattern that can be easily resolved on agarose gel and interpreted by the user. Using this kit after gene editing saves time and energy from the arduous screening process and provides clear genotyping results
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