The 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) Substrate System is an ideal insoluble substrate for use with alkaline phosphatase. This system produces a blue-purple product. The intense color can be observed visually, is very stable, and will not fade upon exposure...
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The 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) Substrate System is an ideal insoluble substrate for use with alkaline phosphatase. This system produces a blue-purple product. The intense color can be observed visually, is very stable, and will not fade upon exposure to light. The BCIP/NBT Substrate System offers a convenient, ready-to-use substrate solution for visualizing alkaline phosphatase activity in immunoblotting and immunohistology. Immunohistochemical staining with BCIP/NBT requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green. BCIP/NBT is generally a more sensitive method than Fast Red
BCIP/NBT Solution is formulated to precipitate and localize the blue-purple reaction product onto membrane surfaces or tissue samples at sites where the alkaline phosphatase-substrate reaction occurs. When performing immunohistochemical techniques, organic mounting media must be used
This product is manufactured by TriBio Science and sold through ABMIUM without relabelling.
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