Annexin V-mCherry Apoptosis Assay Kit

Annexin V belongs to the annexin family of intracellular proteins that bind to phosphatidylserine (PS) in a calcium dependent manner. PS is located on the inner layer of the phospholipid bilayer of the plasma membrane in viable cells. Cells undergoing early apoptosis will transfer PS to the external layer while maintaining an intact plasma membrane. Fluorescently labeled Annexin V may be used to bind the PS specifically and identify cells engaged in the early stages of apoptosis. During late apoptosis or necrosis, plasma membrane integrity is lost, which allows for the access of live/dead cell differential dyes such as 7-ADD, Propidium Iodide, or DRAQ7. The staining of cells with Annexin V will effectively resolve viable apoptotic cell populations by flow cytometry

Fig. 2: Jurkat cells (T cell leukemia, human) are induced by 1.25ug/ml puromycin at 2 hours (upper right), 4 hours (lower left), and 6 hours (lower right) and the control (upper left) which is not treated with puromycin. Then all of them are stained by Annexin V-mCherry and analyzed by flow cytometry. The percentages of Aennix V-mCherry positive cells among live Jurkat cells are shown on the tops of each sample picture. Apoptotic cells are increased after puromycin inductions, and the percentiles of apoptotic cells are higher with the treatment time extensions