Manufactured by:Reed Biotech
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Mouse IL-6 VeriQuant Immunoassay Kit

Mouse IL-6 VeriQuant Immunoassay Kit from Reed Biotech

SKU:
ABM223626-1
Manufacturer SKU
VQ3186M
Product type
Proximity-based Immunoassay
TargetIL-6
Species / reactivityMouse
Sample typeSerum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates, Other biological fluids

Manufacturer provenance

Manufactured by:
Reed Biotech
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

Test principle

(a) Two monoclonal antibodies against distinct epitopes are conjugated with oligo probes, then form a sandwich complex with the antigen. (b) Following antigen recognition by the paired antibodies, the conjugated oligonucleotides are spatially proximate, and generate an amplifiable dsDNA template under the enzymatic action. (c) qPCR amplification is then performed. CT values correlate inversely with protein concentration, and sample target concentrations are calculated via standard curve.
Key facts & specifications
Also known as
B-cell differentiation factor, B-cell stimulatory factor 2, BSF2, BSF-2, CDF, CTL differentiation factor, HSF, hybridoma growth factor, IFNB2, IFN-beta-2, IL6, IL-6, Interferon beta-2, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6, MGI-2A
Assay type
Proximity-based Immunoassay
Detection method
qPCR
Sample volume
1~5μL
Assay instrument
qPCR Instruments
Manual handling time
20 min
Wash-free workflow
Yes
Signal label / dye
FAM™
Sensitivity
0.079pg/mL
Detection range
0.32-5000 pg/mL
Wells / tests
96T
Assay duration
2 H
Quantity per pack
96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Manufacturer-listed assay compatibility

Evidence status by application and model.

Species / target matrixProtein quantification
Mouse

Sample type validation

Sample compatibility evidence using the same validation key.

Sample typeSerumPlasmaTissue homogenatesCell lysatesCell culture supernatesOther biological fluids
Sample compatibility
Identifiers & database references
Manufacturer SKU
VQ3186M
Gene ID
16193
UniProt accession
P08505
Storage, stability & shipping
Storage conditions
-20°C
Shipped at
Ultra-low temperature phase-change materials
Long-term storage
-20°C
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Reed Biotech and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Reed Biotech
£360.00£216.00