Phospho-Histone H2A.X (Ser139) / γH2AX Rabbit Monoclonal Antibody

Phosphorylation-site-specific recombinant rabbit monoclonal antibody recognising histone H2A.X phosphorylated at Ser139 (γH2AX), used in DNA damage signalling research.

Target background

H2A.X is a variant of the core histone H2A and contributes to the nucleosome structure around which DNA is organised. Phosphorylation at Ser139 generates γH2AX, a widely studied event in the chromatin response to DNA double-strand breaks. γH2AX can accumulate in nuclear foci at sites associated with DNA damage signalling, making it a useful experimental marker when interpreted alongside appropriate controls. The phospho-Ser139 designation describes the target epitope, not chemical conjugation of this primary antibody.

Also known as

H2AFX, H2AX, H2A.X, H2a/x, histone H2A.X, gamma-H2AX, γH2AX, phospho-H2AX (Ser139), H2AX-pSer139.

Manufacturer specifications

• Host: rabbit; recombinant monoclonal IgG κ; phospho-Ser139 epitope-specific; supplied without a stated reporter conjugate.
• Species reactivity: human, mouse, rat, hamster. Manufacturer applications: WB 1:1,000–1:5,000; IHC 1:2,000–1:8,000; IF 1:200–1:1,000; IP 1:50–1:200; ELISA 1:5,000–1:20,000.
• Purification: Protein A; form: liquid. Immunogen: synthetic phosphorylated peptide corresponding to the target protein.
• Calculated and observed molecular weight: 15 kDa. Stated concentration: approximately 0.3 mg/mL, subject to lot variation. Gene ID: 3014; UniProtKB: P16104.
• Buffer: PBS, 50% glycerol, 0.05% ProClin 300, 0.05% protective protein. Store aliquoted at −20 °C; avoid freeze-thaw cycles.

Manufacturer application evidence

Manufacturer western blot image of 293-cell lysate with an HRP-conjugated goat anti-rabbit secondary antibody. Other listed applications and species are manufacturer-stated; phosphatase-treatment controls were not provided in the referenced datasheet.

Provenance: Original EnkiLife antibody. Manufacturer-supplied data; no claim of ABMIUM independent validation. Research use only. Manufacturer product page · Product datasheet (PDF).