Apaf-1 pAb de lapin de ELK Biotechnology
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L'anticorps polyclonal de lapin Apaf-1 est un anticorps d'ELK Biotechnology destiné aux flux de travail de recherche impliquant APAF1.
Ce gène code une protéine cytoplasmique qui déclenche l'apoptose. Cette protéine contient plusieurs copies du domaine WD-40, un domaine de recrutement de caspases (CARD) et un domaine ATPase (NB-ARC). Lors de la liaison au cytochrome c et au dATP, cette protéine forme un apoptosome oligomérique. L'apoptosome se lie et clive la préproprotéine de la caspase 9, libérant sa forme mature et activée. La caspase 9 activée stimule la cascade de caspases ultérieure qui engage la cellule dans l'apoptose. L'épissage alternatif entraîne plusieurs variants de transcrits codant différentes isoformes. [fourni par RefSeq, juillet 2008],
| Spécificité | L'anticorps polyclonal Apaf-1 détecte les niveaux endogènes de la protéine Apaf-1. |
|---|---|
| Légendes des preuves de validation | Analyse par Western Blot de cellules K562 utilisant l'anticorps polyclonal Apaf-1. L'anticorps secondaire (catalogue n°: RS0002) a été dilué à 1:20000 | Analyse immunohistochimique de foie humain inclus en paraffine, l'anticorps a été dilué à 1:100 | Analyse immunohistochimique de poumon humain inclus en paraffine, l'anticorps a été dilué à 1:100 | Analyse par Western blot de lysat de cellules K562, utilisant l'anticorps APAF1. |
Fully tested in-house by ABMIUM, or supported by an advanced validation report from a partner reviewed by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Evidence status by application and model.
| Espèce | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Humain | |||||||
| Souris | |||||||
| Rat |
This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.
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Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
APAF1 (apoptotic peptidase activating factor 1; Apaf-1) is a core scaffold of the intrinsic apoptosis pathway. Following mitochondrial cytochrome c release, APAF1 assembles the apoptosome and recruits procaspase-9, linking mitochondrial stress to downstream caspase activation.
Biological function: APAF1 binds cytochrome c and ATP or dATP, then oligomerizes into the apoptosome. Through its CARD domain, the complex recruits and activates procaspase-9, which initiates downstream executioner-caspase signalling including caspase-3 activation.
