Recombinant Human IL-8/CXCL8 protein

Background

Interleukin-8 (IL-8) is encoded by the IL8 gene and produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles. There are many receptors capable to bind IL-8, the most affinity to IL-8 are receptors CXCR1, and CXCR2. As a member of the CXC chemokine family, function of IL-8 is the induction of chemotaxis in its target cells, like neutrophil granulocytes, basophils, and T-cells. IL-8 (72 a.a.) has a 5-10 fold higher activity on neutrophil activation, compared to IL-8 (77 a.a.). IL-8 is often associated with inflammation, it has been cited as a proinflammatory mediator in gingivitis and psoriasis

Expression system

Escherichia coli.

Purity

> 96 % by SDS-PAGE and HPLC analyses.

Molecular weight

Approximately 8.9 kDa, a single, non-glycosylated polypeptide chain containing 77 amino acids.

Endotoxin

Less than 1 EU/μg of rHuIL-8, 77a.a./CXCL8 as determined by LAL method.

Form

Sterile Filtered White lyophilized (freeze-dried) powder. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

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