Manufactured by:BT LAB
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Recombinant Protein A/G (C-6His)

Recombinant Protein A/G (C-6His)

SKU:
ABM219184
Manufacturer SKU
CD00148
Product type
Recombinant Proteins
TargetRecombinant Protein A/G (C-6His)
Expression systemEscherichia coli.
Purity> 96 % by SDS-PAGE and RP-HPLC analyses.

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Protein A/G is an excellent tool for purification and detection of mouse monclonal antibodies from IgG subclasses without interference from these other serum proteins. Individual subclasses of mouse monoclonals are most likely to have stronger affinity to this chimeric protein than to either Protein A or Protein G. Binding is less pH-dependent than either Protein A or Protein G alone, occurring well at pH 5-8.

Expression system
Escherichia coli.
Purity
> 96 % by SDS-PAGE and RP-HPLC analyses.
Molecular weight
Approximately 56.9 kDa with 6×His-tag on C-terminus, a single non-glycosylated polypeptide chain containing 513 amino acids.
Endotoxin
Less than 0.1 EU/μg of Protein A/G, His as determined by LAL method.
Form
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation Lyophilized with no additive.

Packaging image shown for reference. Select a size from the available options.

Key facts & specifications
Research areas
Others
Also known as
Protein A/G, His
Molecular weight
Approximately 56.9 kDa with 6×His-tag on C-terminus, a single non-glycosylated polypeptide chain containing 513 amino acids.
Form / buffer
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation Lyophilized with no additive.
Endotoxin level
Less than 0.1 EU/μg of Protein A/G, His as determined by LAL method.
Quantity per pack
1 mg
Technical note
Reconstitution: Dissolve in distilled water or saline.
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSDS-PAGEHPLCLAL
Quality control
Identifiers & database references
Manufacturer SKU
CD00148
Storage, stability & shipping
Storage conditions
-20ºC
Storage buffer
Lyophilized with no additive.
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£143.80£122.00