Recombinant Rat FGF-10/KGF-2 protein

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Recombinant Rat FGF-10/KGF-2 protein

Recombinant Rat FGF-10/KGF-2 protein

SKU:
ABM219153
Manufacturer SKU
CD00127
Product type
Recombinant Proteins
TargetRecombinant Rat FGF-10/KGF-2 protein
Expression systemEscherichia coli.
Purity> 97 % by SDS-PAGE and HPLC analyses.

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

FGF-10 was originally identified from rat embryos by homology-based polymerase chain reaction. Rat FGF-10 shares approximately 95 % amino acid sequence identity with human FGF-10. Among the FGF family members, FGF-10 is most closely related to FGF-7. The expression of FGF-10 transcripts has been shown to be most abundant in the embryo and adult lung. Recombinant FGF-10 preparations have been shown to be mitogenic for epithelial and epidermal cells but not fibroblasts. Based on its in vitro biological activities and in vivo expression pattern, FGF-10 has been proposed to play unique roles in the brain, in lung development, wound healing and limb bud formation.

Expression system
Escherichia coli.
Purity
> 97 % by SDS-PAGE and HPLC analyses.
Molecular weight
Approximately 20.0 kDa, a single non-glycosylated polypeptide chain containing 179 amino acids.
Endotoxin
Less than 1 EU/μg of rRtKGF-2/FGF-10 as determined by LAL method.
Form
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 500 mM NaCl, pH 7.4, 5 % trehalose.

Packaging image shown for reference. Select a size from the available options.

Key facts & specifications
Research areas
Immunology
Also known as
FGF-10/KGF-2, FGF10, Fgf5a,Rat
Source organism
Rat
Molecular weight
Approximately 20.0 kDa, a single non-glycosylated polypeptide chain containing 179 amino acids.
Form / buffer
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 500 mM NaCl, pH 7.4, 5 % trehalose.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using monkey 4MBr-5 cells is less than 120 ng/ml, corresponding to a specific activity of > 8.3 × 103 IU/mg.
Endotoxin level
Less than 1 EU/μg of rRtKGF-2/FGF-10 as determined by LAL method.
Sequence
QALGQDMVSP EATNSSSSSS SSSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS
Quantity per pack
5 ug
Technical note
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSDS-PAGEHPLCLALBioactivity
Quality control
Identifiers & database references
Manufacturer SKU
CD00127
UniProt accession
P70492
Storage, stability & shipping
Storage conditions
-20ºC
Storage buffer
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 500 mM NaCl, pH 7.4, 5 % trehalose.
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
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