Human Zona Pellucida Sperm-binding Protein 3, ZP3 ELISA Kit

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Human Zona Pellucida Sperm-binding Protein 3, ZP3 ELISA Kit

Human Zona Pellucida Sperm-binding Protein 3, ZP3 ELISA Kit

SKU:
ABM218154
Manufacturer SKU
E6422Hu
Product type
ELISA Kits
TargetZP3
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human ZP3 antibody. ZP3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ZP3 Antibody is added and binds to ZP3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ZP3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ZP3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Tags & Cell Markers
Also known as
OOMD3;Processed zona pellucida sperm-binding protein 3];Sperm receptor;Zona pellucida glycoprotein 3;Zona pellucida protein C;Zona pellucida sperm-binding protein 3;ZP3;Zp-3;ZP3A;ZP3A/ZP3B;ZP3B;ZPC
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
9.73ng/L
Detection range
20-4500ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
47,018 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E6422Hu
Gene symbol
ZP3
Gene ID
7784
UniProt accession
P21754
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00