Human Melanocyte Protein Pmel, SILV ELISA Kit

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Human Melanocyte Protein Pmel, SILV ELISA Kit

Human Melanocyte Protein Pmel, SILV ELISA Kit

SKU:
ABM217012
Manufacturer SKU
E5652Hu
Product type
ELISA Kits
TargetSILV
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human SILV antibody. SILV present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SILV Antibody is added and binds to SILV in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SILV antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SILV. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
95 kDa melanocyte-specific secreted glycoprotein;D12S53E;gp100;M-alpha;M-beta];ME20;ME20M;ME20-M;ME20S;ME20-S;Melanocyte protein PMEL;Melanocyte protein Pmel 17;Melanocytes lineage-specific antigen GP100;Melanoma-associated ME20 antigen;P1;P100;P26;PMEL17;Premelanosome protein;Secreted melanoma-associated ME20 antigen;SI;SIL;SILV;Silver locus protein homolog
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
7.41ng/L
Detection range
15-3000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
70,255 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E5652Hu
Gene symbol
PMEL
Gene ID
6490
UniProt accession
P40967
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00