Manufactured by:BT LAB
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Human Interleukin-18 Receptor 1, IL18R1 ELISA Kit

Human Interleukin-18 Receptor 1, IL18R1 ELISA Kit

SKU:
ABM216788
Manufacturer SKU
E5514Hu
Product type
ELISA Kits
TargetIL18R1
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Within the IL18 receptor complex, responsible for the binding of the proinflammatory cytokine IL18, but not IL1A nor IL1B (PubMed: 8626725, PubMed: 14528293, PubMed: 25261253, PubMed: 25500532). Involved in IL18-mediated IFNG synthesis from T-helper 1 (Th1) cells (PubMed: 10653850). Contributes to IL18-induced cytokine production, either independently of SLC12A3, or as a complex with SLC12A3 (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human IL18R1 antibody. IL18R1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IL18R1 Antibody is added and binds to IL18R1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL18R1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human IL18R1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
CD antigen CD218a;CD218 antigen-like family member A;CDw218a;IL 18R1;IL1 receptor-related protein;IL18R1;IL-18R1;IL-18R-1;IL-18Ralpha;IL-18R-alpha;IL1R-rp;IL-1Rrp;Interleukin-18 receptor 1;Interleukin-18 receptor alpha
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.026ng/ml
Detection range
0.05-20ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
62,304 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E5514Hu
Gene symbol
IL18R1
Gene ID
8809
UniProt accession
Q13478
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

IL18R1interleukin 18 receptor 1

View target guide

IL18R1 identifies interleukin 18 receptor 1 in Homo sapiens (Human). This target record connects NCBI Gene 8809, UniProtKB Q13478 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
8809
UniProtKB
Q13478
BT LAB
£240.93£205.00