Human Gamma-secretase Subunit Aph-1A, APH1A ELISA Kit

BT LAB manufacturer product image

100%
Manufactured by:BT LAB
Save 15%RUO

Human Gamma-secretase Subunit Aph-1A, APH1A ELISA Kit

Human Gamma-secretase Subunit Aph-1A, APH1A ELISA Kit

SKU:
ABM216474
Manufacturer SKU
E5319Hu
Product type
ELISA Kits
TargetAPH1A
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Non-catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed: 12297508, PubMed: 12522139, PubMed: 12763021, PubMed: 12679784, PubMed: 25043039, PubMed: 26280335, PubMed: 30598546, PubMed: 30630874). Required for normal gamma-secretase assembly (PubMed: 12522139, PubMed: 12471034, PubMed: 12763021, PubMed: 19369254). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels (Probable).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human APH1A antibody. APH1A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human APH1A Antibody is added and binds to APH1A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated APH1A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human APH1A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
APH1A;APH-1a;Aph-1alpha;Gamma-secretase subunit APH-1A;Presenilin-stabilization factor
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
9.32ng/L
Detection range
20-3800ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
28,996 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E5319Hu
Gene symbol
APH1A
Gene ID
51107
UniProt accession
Q96BI3
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00