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Human Extracellular Matrix Protein 1, ECM1 ELISA Kit

Human Extracellular Matrix Protein 1, ECM1 ELISA Kit

SKU:
ABM216398
Manufacturer SKU
E5270Hu
Product type
ELISA Kits
TargetECM1
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human ECM1 antibody. ECM1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ECM1 Antibody is added and binds to ECM1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ECM1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ECM1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cardiovascular
Also known as
ECM 1;ECM1;Extracellular matrix protein 1;Secretory component p85
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
15.23ng/L
Detection range
30-9000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
60,674 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E5270Hu
Gene symbol
ECM1
Gene ID
1893
UniProt accession
Q16610
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

ECM1extracellular matrix protein 1

View target guide

ECM1 identifies extracellular matrix protein 1 in Homo sapiens (Human). This target record connects NCBI Gene 1893, UniProtKB Q16610 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
1893
UniProtKB
Q16610
BT LAB
£240.93£205.00