Human Chromatin Assembly Factor 1 Subunit B, CHAF1B ELISA Kit

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Human Chromatin Assembly Factor 1 Subunit B, CHAF1B ELISA Kit

Human Chromatin Assembly Factor 1 Subunit B, CHAF1B ELISA Kit

SKU:
ABM216080
Manufacturer SKU
E5048Hu
Product type
ELISA Kits
TargetCHAF1B
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human CHAF1B antibody. CHAF1B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CHAF1B Antibody is added and binds to CHAF1B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CHAF1B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CHAF1B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
CAF-1 subunit B;CAF-I 60 kDa subunit;CAF-I p60;CHAF 1B;CHAF1B;Chromatin assembly factor 1 subunit B;Chromatin assembly factor I p60 subunit;M-phase phosphoprotein 7
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
10.05ng/L
Detection range
20-3800ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
61,493 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E5048Hu
Gene symbol
CHAF1B
Gene ID
8208
UniProt accession
Q13112
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

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