Manufactured by:BT LAB
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Human Cd27 Antigen, CD27 ELISA Kit

Human Cd27 Antigen, CD27 ELISA Kit

SKU:
ABM216026
Manufacturer SKU
E5018Hu
Product type
ELISA Kits
TargetCD27
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human CD27 antibody. CD27 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CD27 Antibody is added and binds to CD27 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CD27 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CD27. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
CD antigen CD27;CD27;CD27 antigen;CD27L receptor;S152;S152. LPFS2;T14;T-cell activation antigen CD27;TNFRSF7;Tp55;Tumor necrosis factor receptor superfamily member 7
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
1.33ng/mL
Detection range
3-900ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
29,137 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E5018Hu
Gene symbol
CD27
Gene ID
939
UniProt accession
P26842
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CD27CD27 molecule

View target guide

CD27 molecule (CD27), also known as TNFRSF7, is a TNF-receptor-superfamily costimulatory receptor involved in adaptive immune signalling. ABMIUM catalogue products linked below support research on CD27/TNFRSF7 biology.

Biological function: CD27 molecule (CD27/TNFRSF7) encodes a TNF-receptor-superfamily costimulatory receptor. Binding of its ligand CD70 supports antigen-specific T-cell activation and expansion and contributes to B-cell activation and immunoglobulin responses, with signalling through pathways including NF-kappaB and JNK.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
939
UniProtKB
P26842
BT LAB
£240.93£205.00