Human Netrin Receptor Unc5D, UNC5D ELISA Kit

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Human Netrin Receptor Unc5D, UNC5D ELISA Kit

Human Netrin Receptor Unc5D, UNC5D ELISA Kit

SKU:
ABM215392
Manufacturer SKU
E4181hu
Product type
ELISA Kits
TargetUNC5D
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Receptor for the netrin NTN4 that promotes neuronal cell survival (By similarity). Plays a role in cell-cell adhesion and cell guidance. Receptor for netrin involved in cell migration. Plays a role in axon guidance by mediating axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding (By similarity). May play a role in apoptosis in response to DNA damage (PubMed: 24691657). It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand (PubMed: 24519068). Mediates cell-cell adhesion via its interaction with FLRT3 on an adjacent cell (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human UNC5D antibody. UNC5D present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human UNC5D Antibody is added and binds to UNC5D in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated UNC5D antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human UNC5D. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
Netrin receptor UNC5D;Protein unc-5 homolog 4;Protein unc-5 homolog D;UNC5D
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.046ng/ml
Detection range
0.1-40ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
105,880 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E4181hu
Gene symbol
UNC5D
Gene ID
137970
UniProt accession
Q6UXZ4
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00