Human Aquaporin 10, AQP-10 ELISA Kit

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Human Aquaporin 10, AQP-10 ELISA Kit

Human Aquaporin 10, AQP-10 ELISA Kit

SKU:
ABM215352
Manufacturer SKU
E4146hu
Product type
ELISA Kits
TargetAQP10
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

[Isoform 1]: Water Channel that mediates water transport across cell membranes irrespective of the cytosolic pH (PubMed: 1208$458.00|$300.001, PubMed: 21733844, PubMed: 23382902, PubMed: 30420639). The Channel is permeable to glycerol, especially when the cytosolic pH is acidified (PubMed: 21733844, PubMed: 30420639). Contributes to adipocyte water and glycerol permeability, and may thereby contribute to the utilization of glycerol derived from phospholipid degradation (PubMed: 23382902). May contribute to water transport in the intestine (Probable). [Isoform 2]: Water Channel that mediates water transport across cell membranes, but that is not permeable to glycerol.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human AQP10 antibody. AQP10 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human AQP10 Antibody is added and binds to AQP10 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated AQP10 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human AQP10. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
AQP 10;AQP10;AQP-10;Aquaglyceroporin-10;Aquaporin-10;Small intestine aquaporin
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.01ng/ml
Detection range
0.03-10ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
31,763 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E4146hu
Gene symbol
AQP10
Gene ID
89872
UniProt accession
Q96PS8
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00