Manufactured by:BT LAB
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Human Fetuin B, FETU-B ELISA Kit

Human Fetuin B, FETU-B ELISA Kit

SKU:
ABM214642
Manufacturer SKU
E3282Hu
Product type
ELISA Kits
TargetFETUB
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Protease inhibitor required for egg fertilization. Required to prevent premature zona pellucida hardening before fertilization, probably by inhibiting the protease activity of ASTL, a protease that mediates the cleavage of ZP2 and triggers zona pellucida hardening (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human FETUB antibody. FETUB present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human FETUB Antibody is added and binds to FETUB in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated FETUB antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human FETUB. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cardiovascular
Also known as
16G2;FETUB;Fetuin-B;Fetuin-like protein IRL685;Gugu
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
2.38ng/ml
Detection range
5-1500ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
42,055 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E3282Hu
Gene symbol
FETUB
Gene ID
26998
UniProt accession
Q9UGM5
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

FETUBfetuin B

View target guide

FETUB identifies fetuin B in Homo sapiens (Human). This target record connects NCBI Gene 26998, UniProtKB Q9UGM5 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
26998
UniProtKB
Q9UGM5
BT LAB
£240.93£205.00