Manufactured by:BT LAB
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Human Interleukin 31, IL-31 ELISA Kit

Human Interleukin 31, IL-31 ELISA Kit

SKU:
ABM214604
Manufacturer SKU
E3254Hu
Product type
ELISA Kits
TargetIL31
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR (PubMed: 15184896). May function in skin immunity (PubMed: 15184896). Enhances myeloid progenitor cell survival in vitro (By similarity). Induces RETNLA and serum amyloid A protein expression in macrophages (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human IL31 antibody. IL31 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IL31 Antibody is added and binds to IL31 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL31 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human IL31. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
IL 31;IL31;IL-31;Interleukin-31
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
1.2ng/L
Detection range
2-600ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
18,205 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E3254Hu
Gene symbol
IL31
Gene ID
386653
UniProt accession
Q6EBC2
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

IL31interleukin 31

View target guide

IL31 identifies interleukin 31 in Homo sapiens (Human). This target record connects NCBI Gene 386653, UniProtKB Q6EBC2 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
386653
UniProtKB
Q6EBC2
BT LAB
£240.93£205.00