Human Carbohydrate Sulfotransferase 7, CHST7 ELISA Kit

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Human Carbohydrate Sulfotransferase 7, CHST7 ELISA Kit

Human Carbohydrate Sulfotransferase 7, CHST7 ELISA Kit

SKU:
ABM214168
Manufacturer SKU
E2876Hu
Product type
ELISA Kits
TargetCHST7
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues. Preferentially acts on mannose-linked GlcNAc. Also able to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Also acts on core 2 mucin-type oligosaccharide and N-acetyllactosamine oligomer with a lower efficiency. Has weak or no activity toward keratan sulfate and oligosaccharides containing the Galbeta1-4GlcNAc. Catalyzes 6-O-sulfation of beta-benzyl GlcNAc but not alpha- or beta-benzyl GalNAc.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human CHST7 antibody. CHST7 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CHST7 Antibody is added and binds to CHST7 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CHST7 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CHST7. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction
Also known as
C6ST-2;Carbohydrate sulfotransferase 7;Chondroitin 6-sulfotransferase 2;CHST7;Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 5;GlcNAc6ST-4;Gn6st-4;GST-5;N-acetylglucosamine 6-O-sulfotransferase 4;
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.021ng/ml
Detection range
0.05-15ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
54,266 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2876Hu
Gene symbol
CHST7
Gene ID
56548
UniProt accession
Q9NS84
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
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