Manufactured by:BT LAB
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Mouse Cystathionine Beta-synthase, CBS ELISA Kit

Mouse Cystathionine Beta-synthase, CBS ELISA Kit

SKU:
ABM213920
Manufacturer SKU
E2703Mo
Product type
ELISA Kits
TargetCBS
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine (By similarity). Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse CBS antibody. CBS present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse CBS Antibody is added and binds to CBS in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CBS antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse CBS. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
Beta-thionase;CBS;Cystathionine beta-synthase;Serine sulfhydrase
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.24ng/ml
Detection range
0.5-100ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
61,544 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2703Mo
Gene symbol
CBS
Gene ID
12411
UniProt accession
Q91WT9
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CBScystathionine beta-synthase

View target guide

CBS identifies cystathionine beta-synthase in Homo sapiens (Human). This target record connects NCBI Gene 875, UniProtKB P35520 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
875
UniProtKB
P35520
BT LAB
£240.93£205.00