Manufactured by:BT LAB
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Mouse Sparc-like Protein 1, SPARCL1 ELISA Kit

Mouse Sparc-like Protein 1, SPARCL1 ELISA Kit

SKU:
ABM213322
Manufacturer SKU
E2484Mo
Product type
ELISA Kits
TargetSPARCL1
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

SPARC-like protein 1 (SPARCL1 or SC1), also known as hevin (short for high endothelial venule protein), is a secreted protein with high structural similarity to SPARC. It interacts with the extracellular matrix to create intermediate states of cell adhesion. Due to its dynamic extracellular roles, being implicated in cancer metastasis and inflammation, it is considered a matricellular protein. In humans hevin is encoded by the SPARCL1 gene.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse SPARCL1 antibody. SPARCL1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse SPARCL1 Antibody is added and binds to SPARCL1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SPARCL1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse SPARCL1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
Ecm2;Extracellular matrix protein 2;he;hevin;mas;Matrix glycoprotein Sc1;Sc;Sc1;SPARCL1;SPARC-like protein 1
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
14.94ng/L
Detection range
30-9000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
72,287 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2484Mo
Gene symbol
Sparcl1
Gene ID
13602
UniProt accession
P70663
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

SPARCL1SPARC like 1

View target guide

SPARCL1 identifies SPARC like 1 in Homo sapiens (Human). This target record connects NCBI Gene 8404, UniProtKB Q14515 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
8404
UniProtKB
Q14515
BT LAB
£240.93£205.00