Mouse Multidrug Resistance Protein 3, ABCB4 ELISA Kit

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Mouse Multidrug Resistance Protein 3, ABCB4 ELISA Kit

Mouse Multidrug Resistance Protein 3, ABCB4 ELISA Kit

SKU:
ABM212544
Manufacturer SKU
E2270Mo
Product type
ELISA Kits
TargetABCB1B
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Translocates drugs and phospholipids across the membrane. Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins. Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse ABCB1B antibody. ABCB1B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse ABCB1B Antibody is added and binds to ABCB1B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ABCB1B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse ABCB1B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
ABCB1B;ATP-binding cassette sub-family B member 1B;ATP-dependent translocase ABCB1;CD antigen CD243;Multidrug resistance protein 1B;P-glycoprotein 1;Phospholipid transporter ABCB1
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
9.07ng/L
Detection range
20-3500ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
140,994 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2270Mo
Gene symbol
ABCB1B
Gene ID
18669
UniProt accession
P06795
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
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