Manufactured by:BT LAB
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Mouse Monocyte Chemotactic Protein 5, MCP-5 ELISA Kit

Mouse Monocyte Chemotactic Protein 5, MCP-5 ELISA Kit

SKU:
ABM212524
Manufacturer SKU
E2266Mo
Product type
ELISA Kits
TargetCCL12
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse CCL12 antibody. CCL12 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse CCL12 Antibody is added and binds to CCL12 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CCL12 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse CCL12. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
C-C motif chemokine 12;CCL 12;CCL12;MCP-1-related chemokine;MCP-5;Monocyte chemoattractant protein 5;Monocyte chemotactic protein 5;Small-inducible cytokine A12
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
5.82ng/L
Detection range
10-2000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
11,659 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2266Mo
Gene symbol
CCL12
Gene ID
20293
UniProt accession
Q62401
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

Ccl12C-C motif chemokine ligand 12

View target guide

Ccl12 identifies C-C motif chemokine ligand 12 in Mus musculus (House Mouse). This target record connects NCBI Gene 20293, UniProtKB Q62401 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Mus musculus (House Mouse)
NCBI Gene
20293
UniProtKB
Q62401
BT LAB
£240.93£205.00