Manufactured by:BT LAB
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Mouse Mesoderm-specific Transcript Homolog Protein, MEST ELISA Kit

Mouse Mesoderm-specific Transcript Homolog Protein, MEST ELISA Kit

SKU:
ABM212426
Manufacturer SKU
E2247Mo
Product type
ELISA Kits
TargetMEST
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Mesoderm-specific transcript homolog protein is a protein that in humans is encoded by the MEST gene. This gene encodes a member of the Alpha/beta hydrolase superfamily and has isoform-specific imprinting. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promoter switching. The encoded protein may play a role in development. Three transcript variants encoding two distinct isoforms have been identified for this gene. A pseudogene for this locus is located on chromosome 6.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse MEST antibody. MEST present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse MEST Antibody is added and binds to MEST in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MEST antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse MEST. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
AA408879;AI256745;Mesoderm-specific transcript protein;MEST;Paternally-expressed gene 1 protein;Peg;Peg1
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
9.34ng/L
Detection range
20-4500ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
38,907 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2247Mo
Gene symbol
Mest
Gene ID
17294
UniProt accession
Q07646
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00