Manufactured by:BT LAB
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Mouse Interleukin-34, IL34 ELISA Kit

Mouse Interleukin-34, IL34 ELISA Kit

SKU:
ABM212110
Manufacturer SKU
E2190Mo
Product type
ELISA Kits
TargetIL34
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1 (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse IL34 antibody. IL34 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse IL34 Antibody is added and binds to IL34 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL34 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse IL34. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
IL 34;IL34;IL-34;Interleukin-34
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.27ng/L
Detection range
0.5-200ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
26,764 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2190Mo
Gene symbol
IL34
Gene ID
76527
UniProt accession
Q8R1R4
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

IL34interleukin 34

View target guide

IL34 identifies interleukin 34 in Homo sapiens (Human). This target record connects NCBI Gene 146433, UniProtKB Q6ZMJ4 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
146433
UniProtKB
Q6ZMJ4
BT LAB
£240.93£205.00