Manufactured by:BT LAB
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Mouse Cystatin-A, CSTA ELISA Kit

Mouse Cystatin-A, CSTA ELISA Kit

SKU:
ABM211466
Manufacturer SKU
E1989Mo
Product type
ELISA Kits
TargetCSTA
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Cystatin-A is a protein that in humans is encoded by the CSTA gene. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse CSTA antibody. CSTA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse CSTA Antibody is added and binds to CSTA in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CSTA antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse CSTA. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cell Biology
Also known as
Cs;Csta;Cystatin-A;Cystatin-A1;Stefin-A
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
14.17ng/L
Detection range
30-9000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
10,929 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1989Mo
Gene symbol
Csta
Gene ID
209294
UniProt accession
P56567
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CSTAcystatin A

View target guide

CSTA identifies cystatin A in Homo sapiens (Human). This target record connects NCBI Gene 1475, UniProtKB P01040 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
1475
UniProtKB
P01040
BT LAB
£240.93£205.00