Manufactured by:BT LAB
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Rat Coagulation Factor 7, F7 ELISA Kit

Rat Coagulation Factor 7, F7 ELISA Kit

SKU:
ABM211238
Manufacturer SKU
E1914Ra
Product type
ELISA Kits
TargetF7
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat F7 antibody. F7 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat F7 Antibody is added and binds to F7 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated F7 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat F7. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Blood Coagulation
Also known as
Coagulation factor VII;F7;Factor VII heavy chain;Factor VII light chain;Serum prothrombin conversion accelerator
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.147ng/mL
Detection range
0.3-90ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
50399 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1914Ra
Gene symbol
F7
Gene ID
260320
UniProt accession
Q8K3U6
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

F7coagulation factor VII

View target guide

F7 identifies coagulation factor VII in Homo sapiens (Human). This target record connects NCBI Gene 2155, UniProtKB P08709 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
2155
UniProtKB
P08709
BT LAB
£240.93£205.00