Manufactured by:BT LAB
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Rat Aminopeptidase N, ANPEP ELISA Kit

Rat Aminopeptidase N, ANPEP ELISA Kit

SKU:
ABM211056
Manufacturer SKU
E1858Ra
Product type
ELISA Kits
TargetANPEP
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Broad specificity aminopeptidase which plays a role in the final digestion of Peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various Peptides including Peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of Peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization. May have a role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 and regulating its activity (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat ANPEP antibody. ANPEP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat ANPEP Antibody is added and binds to ANPEP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ANPEP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat ANPEP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology, Stem Cells
Also known as
Alanyl aminopeptidase;Aminopeptidase M;Aminopeptidase N;ANPEP;AP-M;AP-N;CD antigen CD13;CD13;Kidney Zn peptidase;KZP;LAP1;Microsomal aminopeptidase;PEPN;rAPN
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.523ng/mL
Detection range
0.1-40ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
109,449 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1858Ra
Gene symbol
ANPEP
Gene ID
81641
UniProt accession
P15684
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

ANPEPalanyl aminopeptidase, membrane

View target guide

ANPEP identifies alanyl aminopeptidase, membrane in Homo sapiens (Human). This target record connects NCBI Gene 290, UniProtKB P15144 and related ABMIUM catalogue products.

Biological function: Alanyl aminopeptidase, membrane (ANPEP; aminopeptidase N/CD13) is a zinc-dependent ectopeptidase that removes N-terminal neutral amino acids from peptides. It participates in peptide processing and is widely used as a surface marker in myeloid and other cell populations.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
290
UniProtKB
P15144
BT LAB
£240.93£205.00