Manufactured by:BT LAB
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Rat Mitochondrial Uncoupling Protein 2, UCP2 ELISA Kit

Rat Mitochondrial Uncoupling Protein 2, UCP2 ELISA Kit

SKU:
ABM210822
Manufacturer SKU
E1766Ra
Product type
ELISA Kits
TargetUCP2
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat UCP2 antibody. UCP2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat UCP2 Antibody is added and binds to UCP2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated UCP2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat UCP2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
Mitochondrial uncoupling protein 2;Solute carrier family 25 member 8;UCP 2;UCP2
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
5.03ng/L
Detection range
9-2000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
33,377 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1766Ra
Gene symbol
UCP2
Gene ID
54315
UniProt accession
P56500
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

UCP2uncoupling protein 2

View target guide

UCP2 identifies uncoupling protein 2 in Homo sapiens (Human). This target record connects NCBI Gene 7351, UniProtKB P55851 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
7351
UniProtKB
P55851
BT LAB
£240.93£205.00