Manufactured by:BT LAB
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Rat Angiopoietin-like Protein 2, ANGPTL2 ELISA Kit

Rat Angiopoietin-like Protein 2, ANGPTL2 ELISA Kit

SKU:
ABM210396
Manufacturer SKU
E1504Ra
Product type
ELISA Kits
TargetAMOTL2
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Regulates the translocation of phosphorylated SRC to peripheral cell-matrix adhesion sites. Required for proper architecture of actin filaments. Inhibits the Wnt/beta-catenin signaling pathway, probably by recruiting CTNNB1 to recycling endosomes and hence preventing its translocation to the nucleus. Participates in angiogenesis. May play a role in the polarity, proliferation and migration of endothelial cells. Selectively promotes FGF-induced MAPK activation through SRC (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat AMOTL2 antibody. AMOTL2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat AMOTL2 Antibody is added and binds to AMOTL2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated AMOTL2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat AMOTL2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cell Biology
Also known as
AMOTL 2;AMOTL2;Angiomotin-like protein 2
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
5.29ng/L
Detection range
10-3500ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
85,587 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1504Ra
Gene symbol
AMOTL2
Gene ID
65157
UniProt accession
G3V735
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00