Manufactured by:BT LAB
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Human Fatty Acid Binding Protein 5, FABP5 ELISA Kit

Human Fatty Acid Binding Protein 5, FABP5 ELISA Kit

SKU:
ABM210184
Manufacturer SKU
E1399Hu
Product type
ELISA Kits
TargetFABP5
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Intracellular carrier for long-chain fatty acids and related active lipids, such as the endocannabinoid, that regulates the metabolism and actions of the ligands they bind. In addition to the cytosolic transport, selectively delivers specific fatty acids from the cytosol to the nucleus, wherein they activate nuclear receptors (PubMed: 22170058, PubMed: 21395585). Delivers retinoic acid to the nuclear receptor peroxisome proliferator-activated receptor delta; which promotes proliferation and survival. May also serve as a synaptic carrier of endocannabinoid at central synapses and thus controls retrograde endocannabinoid signaling. Modulates inflammation by regulating PTGES induction via NF-kappa-B activation, and prostaglandin E2 (PGE2) biosynthesis during inflammation (By similarity). May be involved in keratinocyte differentiation (PubMed: 8092987).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human FABP5 antibody. FABP5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human FABP5 Antibody is added and binds to FABP5 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated FABP5 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human FABP5. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction
Also known as
E-FABP;Epidermal-type fatty acid-binding protein;FABP 5;FABP5;Fatty acid-binding protein 5;Fatty acid-binding protein, epidermal;PA-FABP;Psoriasis-associated fatty acid-binding protein homolog
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.091ng/ml
Detection range
0.2-60ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
15,164 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1399Hu
Gene symbol
FABP5
Gene ID
2171
UniProt accession
Q01469
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

FABP5fatty acid binding protein 5

View target guide

FABP5 identifies fatty acid binding protein 5 in Homo sapiens (Human). This target record connects NCBI Gene 2171, UniProtKB Q01469 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
2171
UniProtKB
Q01469
BT LAB
£240.93£205.00