Manufactured by:BT LAB
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Rat Follistatin-related Protein 3, FSTL3 ELISA Kit

Rat Follistatin-related Protein 3, FSTL3 ELISA Kit

SKU:
ABM209414
Manufacturer SKU
E0890Ra
Product type
ELISA Kits
TargetFSTL3
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

The secreted form is a binding and antagonizing protein for members of the TGF-beta family, such us activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling; more effective on activin A than on activin B. Involved in bone formation; inhibits osteoclast differentiation. Involved in hematopoiesis; involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. The nuclear form is probably involved in transcriptional regulation via interaction with MLLT10 (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat FSTL3 antibody. FSTL3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat FSTL3 Antibody is added and binds to FSTL3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated FSTL3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat FSTL3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
Follistatin-like protein 3;Follistatin-related gene protein;Follistatin-related protein 3;FSTL 3;FSTL3
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.218ng/mL
Detection range
0.5-150ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
27,109 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0890Ra
Gene symbol
FSTL3
Gene ID
114031
UniProt accession
Q99PW7
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

FSTL3follistatin like 3

View target guide

FSTL3 identifies follistatin like 3 in Homo sapiens (Human). This target record connects NCBI Gene 10272, UniProtKB O95633 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
10272
UniProtKB
O95633
BT LAB
£240.93£205.00