Manufactured by:BT LAB
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Mouse Caspase 12, CASP12 ELISA Kit

Mouse Caspase 12, CASP12 ELISA Kit

SKU:
ABM209262
Manufacturer SKU
E0781Mo
Product type
ELISA Kits
TargetCASP12
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Caspase 12 is a protein that in humans is encoded by the CASP12 gene. The protein belongs to a family of enzymes called caspases which cleave their substrates at C-terminal aspartic acid residues. It is closely related to caspase 1 and other members of the caspase family, known as inflammatory caspases, which process and activate inflammatory cytokines such as interleukin 1 and interleukin 18.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse CASP12 antibody. CASP12 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse CASP12 Antibody is added and binds to CASP12 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CASP12 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse CASP12. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Neuroscience, Cell Biology, Cancer, Metabolism
Also known as
CASP12;CASP-12;Caspase-12
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.026ng/ml
Detection range
0.05-20ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
47,854 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0781Mo
Gene symbol
Casp12
Gene ID
12364
UniProt accession
O08736
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00