Manufactured by:BT LAB
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Rat Oncostatin-M, OSM ELISA Kit

Rat Oncostatin-M, OSM ELISA Kit

SKU:
ABM209172
Manufacturer SKU
E0726Ra
Product type
ELISA Kits
TargetOSM
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Growth regulator. Inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells (By similarity). Uses only type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat OSM antibody. OSM present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat OSM Antibody is added and binds to OSM in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated OSM antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat OSM. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
Oncostatin-M;OSM
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
5.34ng/L
Detection range
10-2000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
27,106 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0726Ra
Gene symbol
OSM
Gene ID
289747
UniProt accession
Q65Z15
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

OSMoncostatin M

View target guide

OSM identifies oncostatin M in Homo sapiens (Human). This target record connects NCBI Gene 5008, UniProtKB P13725 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
5008
UniProtKB
P13725
BT LAB
£240.93£205.00