Manufactured by:BT LAB
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Mouse P16 ELISA Kit

Mouse P16 ELISA Kit

SKU:
ABM209000
Manufacturer SKU
E0628Mo
Product type
ELISA Kits
TargetP16ink4a
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse P16ink4a antibody. P16ink4a present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse P16ink4a Antibody is added and binds to P16ink4a in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated P16ink4a antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse P16ink4a. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cell Biology, Epigenetics and Nuclear Signaling, Cancer
Also known as
Arf;ARF-INK4a;CDK4I;Cyclin-dependent kinase 4 inhibitor A;Cyclin-dependent kinase inhibitor 2A;INK4;Ink4a/Arf;INK4a-ARF;MTS;MTS1;p1;p16;p16(INK4a);p16I;p16-INK4;p16INK4a;p16-INK4a;p19;p19 ;p19ARF;Pct;Pctr1
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
1.02ng/L
Detection range
2-600ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
17,941 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0628Mo
Gene symbol
Cdkn2a
Gene ID
12578
UniProt accession
P51480
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CDKN2Acyclin dependent kinase inhibitor 2A

View target guide

CDKN2A identifies cyclin dependent kinase inhibitor 2A in Homo sapiens (Human). This target record connects NCBI Gene 1029, UniProtKB P42771 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
1029
UniProtKB
P42771
BT LAB
£240.93£205.00