Manufactured by:BT LAB
Save 15%RUO

Canine Beta2-Microglobulin, BMG, BETA2-MG ELISA Kit

Canine Beta2-Microglobulin, BMG, BETA2-MG ELISA Kit

SKU:
ABM208592
Manufacturer SKU
E0377Ca
Product type
ELISA Kits
TargetBMG
ApplicationsELISA
Species / reactivityCanine
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Canine BMG antibody. BMG present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine BMG Antibody is added and binds to BMG in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated BMG antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Canine BMG. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
Beta-2-microglobulin;BMG;Fragment
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.19μg/mL
Detection range
0.3-90ug/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
4,842 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0377Ca
Gene symbol
B2M
Gene ID
100855741
UniProt accession
P19341
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

B2Mbeta-2-microglobulin

View target guide

Beta-2-microglobulin (B2M, beta2-m) is the non-polymorphic light-chain component of major histocompatibility complex class I molecules. ABMIUM catalogue products linked below support research on B2M biology.

Biological function: Beta-2-microglobulin (B2M/beta2-m) associates non-covalently with major histocompatibility complex class I heavy chains and is required for stable MHC class I assembly and cell-surface expression. Through this role, B2M is integral to MHC-I antigen presentation and immune recognition by CD8-positive T cells.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
567
UniProtKB
P61769
BT LAB
£263.52£224.00