Manufactured by:BT LAB
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Rabbit Apolipoprotein A1, APO-A1 ELISA Kit

Rabbit Apolipoprotein A1, APO-A1 ELISA Kit

SKU:
ABM207872
Manufacturer SKU
E0149Rb
Product type
ELISA Kits
TargetAPOA1
ApplicationsELISA
Species / reactivityRabbit
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rabbit APOA1 antibody. APOA1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rabbit APOA1 Antibody is added and binds to APOA1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated APOA1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rabbit APOA1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cardiovascular
Also known as
APOA 1;APOA1;ApoA-I;Apo-AI;Apolipoprotein A1;Apolipoprotein A-I;ProapoA-I;Proapolipoprotein A-I;Truncated apolipoprotein A-I;
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
1.21μg/ml
Detection range
2-700ug/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
30,591 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0149Rb
Gene symbol
APOA1
Gene ID
100009253
UniProt accession
P09809
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

APOA1apolipoprotein A1

View target guide

APOA1 encodes apolipoprotein A1; the mature protein apolipoprotein A-I (ApoA-I) is the major protein component of plasma HDL and a central mediator of cholesterol efflux and reverse cholesterol transport.

Biological function: Apolipoprotein A-I promotes export of cellular cholesterol to HDL and transport toward the liver for excretion. ApoA-I also serves as a cofactor for lecithin-cholesterol acyltransferase (LCAT), supporting plasma cholesteryl-ester formation and HDL maturation.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
335
UniProtKB
P02647
BT LAB
£240.93£205.00