Manufactured by:BT LAB
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Human Macrophage Inflammatory Protein-1Α, MIP-1A ELISA Kit

Human Macrophage Inflammatory Protein-1Α, MIP-1A ELISA Kit

SKU:
ABM207828
Manufacturer SKU
E0139Hu
Product type
ELISA Kits
TargetCCL3
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human CCL3 antibody. CCL3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CCL3 Antibody is added and binds to CCL3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CCL3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CCL3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
C-C motif chemokine 3;CCL 3;CCL3;G0/G1 switch regulatory protein 19-1;LD78-alpha(4-69;Macrophage inflammatory protein 1-alpha;MIP-1-alpha;MIP-1-alpha(4-69;PAT 464.1;SIS-beta;Small-inducible cytokine A3;Tonsillar lymphocyte LD78 alpha protein
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.56ng/L
Detection range
1-400ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
10,085 Da
Quantity per pack
96T, 48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0139Hu
Gene symbol
CCL3
Gene ID
6348
UniProt accession
P10147
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CCL3C-C motif chemokine ligand 3

View target guide

CCL3 identifies C-C motif chemokine ligand 3 in Homo sapiens (Human). This target record connects NCBI Gene 6348, UniProtKB P10147 and related ABMIUM catalogue products.

Biological function: CCL3 (MIP-1-alpha) is a secreted CC chemokine involved in inflammatory cell recruitment and immune-cell trafficking. It acts through chemokine receptors including CCR1, CCR4 and CCR5 and can recruit and activate leukocyte populations.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
6348
UniProtKB
P10147
BT LAB
£344.83£293.00