Manufactured by:BT LAB
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Human Monocyte Chemotactic Protein 2, MCP-2 ELISA Kit

Human Monocyte Chemotactic Protein 2, MCP-2 ELISA Kit

SKU:
ABM207758
Manufacturer SKU
E0125Hu
Product type
ELISA Kits
TargetCCL8
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human CCL8 antibody. CCL8 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CCL8 Antibody is added and binds to CCL8 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CCL8 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CCL8. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
C-C motif chemokine 8;CCL 8;CCL8;HC14;MCP-2;MCP-2(6-76;Monocyte chemoattractant protein 2;Monocyte chemotactic protein 2;Small-inducible cytokine A8;
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
9.44ng/L
Detection range
20-3800ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
11,246 Da
Quantity per pack
96T, 48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0125Hu
Gene symbol
CCL8
Gene ID
6355
UniProt accession
P80075
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CCL8C-C motif chemokine ligand 8

View target guide

CCL8 identifies C-C motif chemokine ligand 8 in Homo sapiens (Human). This target record connects NCBI Gene 6355, UniProtKB P80075 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
6355
UniProtKB
P80075
BT LAB
£344.83£293.00