Manufactured by:BT LAB
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Porcine Interleukin 13, IL-13 ELISA Kit

Porcine Interleukin 13, IL-13 ELISA Kit

SKU:
ABM207678
Manufacturer SKU
E0112Po
Product type
ELISA Kits
TargetIL13
ApplicationsELISA
Species / reactivityPorcine
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine IL13 antibody. IL13 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine IL13 Antibody is added and binds to IL13 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL13 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine IL13. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
IL 13;IL13;IL-13;Interleukin-13
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
2.31ng/L
Detection range
5-600ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
14,452 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0112Po
Gene symbol
IL13
Gene ID
396721
UniProt accession
Q95J68
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

IL13interleukin 13

View target guide

IL13 identifies interleukin 13 in Homo sapiens (Human). This target record connects NCBI Gene 3596, UniProtKB P35225 and related ABMIUM catalogue products.

Biological function: IL13 is a cytokine with important roles in allergic inflammation and anti-parasite immunity. It stimulates B-cell proliferation and activates eosinophils, basophils and mast cells, signalling through IL4R/IL13RA1 to activate STAT6.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
3596
UniProtKB
P35225
BT LAB
£240.93£205.00