Manufactured by:BT LAB
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Rabbit Angiotensin Converting Enzyme, ACE ELISA Kit

Rabbit Angiotensin Converting Enzyme, ACE ELISA Kit

SKU:
ABM207366
Manufacturer SKU
E0043Rb
Product type
ELISA Kits
TargetACE
ApplicationsELISA
Species / reactivityRabbit
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rabbit ACE antibody. ACE present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rabbit ACE Antibody is added and binds to ACE in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ACE antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rabbit ACE. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cardiovascular, Signal Transduction, Cell Biology, Cancer, Metabolism
Also known as
ACE;Angiotensin-converting enzyme;Angiotensin-converting enzyme, soluble form;CD antigen CD143;Dipeptidyl carboxypeptidase I;Kininase II
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.33ng/ml
Detection range
0.5-180ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
150,406 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0043Rb
Gene symbol
ACE
Gene ID
100009274
UniProt accession
P12822
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

ACEangiotensin I converting enzyme

View target guide

Angiotensin I converting enzyme (ACE), also known as angiotensin-converting enzyme, ACE1 or CD143, is a peptidase in the renin-angiotensin system. It converts angiotensin I to angiotensin II and inactivates bradykinin, making ACE a central target in blood-pressure, cardiovascular and renal physiology research.

Biological function: Angiotensin I converting enzyme (ACE; ACE1/CD143) is a peptidase in the renin-angiotensin system that converts angiotensin I to angiotensin II and also inactivates bradykinin, linking it to blood-pressure and fluid-electrolyte regulation.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
1636
UniProtKB
P12821
BT LAB
£240.93£205.00