Manufactured by:BT LAB
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Goat Vasoactive Intestinal Peptide, VIP ELISA Kit

Goat Vasoactive Intestinal Peptide, VIP ELISA Kit

SKU:
ABM207354
Manufacturer SKU
E0041GO
Product type
ELISA Kits
TargetVIP
ApplicationsELISA
Species / reactivityGoat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Goat VIP antibody. VIP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Goat VIP Antibody is added and binds to VIP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated VIP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Goat VIP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
Vasoactive intestinal Peptide;Vasoactive intestinal polypeptide;VIP
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.019pmol/L
Detection range
0.05-15pmol/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
3,327 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0041GO
Gene symbol
VIP
Gene ID
102174029
UniProt accession
P63290
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

VIPvasoactive intestinal peptide

View target guide

VIP identifies vasoactive intestinal peptide in Homo sapiens (Human). This target record connects NCBI Gene 7432, UniProtKB P01282 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
7432
UniProtKB
P01282
BT LAB
£240.93£205.00