Monkey C-Peptide, C-P ELISA Kit

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Monkey C-Peptide, C-P ELISA Kit

Monkey C-Peptide, C-P ELISA Kit

SKU:
ABM207296
Manufacturer SKU
E0030Mk
Product type
ELISA Kits
TargetC-P
ApplicationsELISA
Species / reactivityMonkey
Sample typeSerum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue, Cell culture supernatants, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

C-peptide of insulin is a cleavage product produced during processing of the insulin pro-hormone to the mature insulin molecule. Proinsulin is cleaved when it is released from the pancreas into the blood - one C-peptide for each insulin molecule. C-Peptide has been shown to bind to a variety of cell types, and in vivo animal diabetes models have shown positive effects on kidney and nerve function.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Monkey C-P antibody. C-P present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Monkey C-P Antibody is added and binds to C-P in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated C-P antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Monkey C-P. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction, Neuroscience, Metabolism
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
5.09pmol/L
Detection range
10-3000pmol/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaUrineAscitesCerebrospinal fluidTissueCell culture supernatantsTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0030Mk
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8°C
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£271.04£230.00