Manufactured by:BT LAB
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Canine Von Willebrand Factor, VWF ELISA Kit

Canine Von Willebrand Factor, VWF ELISA Kit

SKU:
ABM207252
Manufacturer SKU
E0022Ca
Product type
ELISA Kits
TargetVWF
ApplicationsELISA
Species / reactivityCanine
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex, glycoprotein Ibalpha/IX/V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Canine VWF antibody. VWF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine VWF Antibody is added and binds to VWF in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated VWF antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Canine VWF. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cardiovascular
Also known as
von Willebrand factor;vWF
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.15ng/ml
Detection range
0.3-90ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
309,719 Da
Quantity per pack
96T, 48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0022Ca
Gene symbol
VWF
Gene ID
399544
UniProt accession
Q28295
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

VWFvon Willebrand factor

View target guide

Von Willebrand factor (VWF; also F8VWF) is a multimeric plasma glycoprotein central to primary hemostasis. This ABMIUM target record links human VWF (NCBI Gene 7450; UniProt P04275) to catalogue-backed research products.

Biological function: Von Willebrand factor (VWF) supports platelet adhesion at sites of vascular injury and participates in transport of proteins in blood. Its multimeric structure is central to normal hemostatic function.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
7450
UniProtKB
P04275
BT LAB
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