Manufactured by:BT LAB
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Phospho-C/EBP Alpha (S21) Polyclonal Antibody

Phospho-C/EBP Alpha (S21) Polyclonal Antibody

SKU:
ABM206867
Manufacturer SKU
BT-PHS00597
Product type
Primary Antibodies
TargetCEBPA
ApplicationsWB, IHC, ELISA, IHC-P
Species / reactivityHuman, Mouse, Rat

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

This intronless gene (CEBPA) encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein (CCAAT/enhancer binding protein alpha) functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of CEBPA is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons.

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Key facts & specifications
Research areas
Cardiovascular, Cancer
Also known as
CEBPA; CCAAT/enhancer-binding protein alpha; C/EBP alpha
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB: 1: 500 - 1: 2000; IHC: 1: 100 - 1: 300; ELISA: 1: 5000
Immunogen
The antiserum was produced against synthesized peptide derived from human C/EBP-alpha around the phosphorylation site of Ser21. AA range:6-55
Molecular weight
37575
Concentration
1 mg/ml
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Quantity per pack
20ul, 50ul, 100ul
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceWBIHCELISAIHC-p
Applications
Identifiers & database references
Manufacturer SKU
BT-PHS00597
Gene ID
1050
UniProt accession
P49715
Storage, stability & shipping
Shelf life
-20°C for one year
Storage conditions
-20°C for one year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CEBPACCAAT enhancer binding protein alpha

View target guide

CEBPA identifies CCAAT enhancer binding protein alpha in Homo sapiens (Human). This target record connects NCBI Gene 1050, UniProtKB P49715 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
1050
UniProtKB
P49715
BT LAB
£81.31£69.00