Manufactured by:ELK Biotechnology
RUO

HDAC1 rabbit pAb

HDAC1 rabbit pAb from ELK Biotechnology

SKU:
ABM107126
Product type
Primary Antibody
TargetHDAC1
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemUbiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

HDAC1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying HDAC1 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity HDAC1 Polyclonal Antibody detects endogenous levels of HDAC1 protein.
Validation evidence captions Western Blot analysis of various cells using HDAC1 Polyclonal Antibody diluted at 1:500 | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using HDAC1 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from NIH/3T3 cells, using HDAC1 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from COLO205 cells using HDAC1 antibody.
Key facts & specifications
Research areas
>>Cell cycle, >>Longevity regulating pathway - multiple species, >>Notch signaling pathway, >>Neutrophil extracellular trap formation, >>Thyroid hormone signaling pathway, >>Huntington disease, >>Amphetamine addiction, >>Alcoholism, >>Human papillomavirus infection, >>Epstein-Barr virus infection, >>Pathways in cancer, >>Transcriptional misregulation in cancer, >>Viral carcinogenesis, >>MicroRNAs in cancer, >>Chronic myeloid leukemia
Also known as
HDAC1; RPD3L1; Histone deacetylase 1; HD1
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human HDAC1. AA range:387-436
Observed band
55kD
Cellular localization
Nucleus .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 387-436
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES2498
UniProt entry
Q13547
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

HDAC1histone deacetylase 1

View target guide

HDAC1 identifies histone deacetylase 1 in Homo sapiens (Human). This target record connects NCBI Gene 3065, UniProtKB Q13547 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
3065
UniProtKB
Q13547
ELK Biotechnology
£220.00