Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM95738

Actin β rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Actin β rabbit pAb from ELK Biotechnology Read more ›

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Product Description

Actin β rabbit pAb is a research-use antibody from ELK Biotechnology for studying Actin β in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat, Chicken, Globefish, Bovine, Hamster, Pig, Ovine, Cat, Dog. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Actin β Polyclonal Antibody detects endogenous levels of Actin β protein.

Key Facts

Product typePrimary Antibody Target: Actin β
Collaborator ELK Biotechnology
Partner model ES1592
Target Actin β
Research areas>>Rap1 signaling pathway, >>Phagosome, >>Apoptosis, >>Hippo signaling pathway, >>Focal adhesion, >>Adherens junction, >>Tight junction, >>Platelet activation, >>Neutrophil extracellular trap formation, >>Leukocyte transendothelial migration, >>Thermogenesis, >>Regulation of actin cytoskeleton, >>Thyroid hormone signaling pathway, >>Oxytocin signaling pathway, >>Gastric acid secretion, >>Amyotrophic lateral sclerosis, >>Bacterial invasion of epithelial cells, >>Vibrio cholerae infection, >>Pathogenic Escherichia coli infection, >>Shigellosis, >>Salmonella infection, >>Yersinia infection, >>Influenza A, >>Proteoglycans in cancer, >>Hepatocellular carcinoma, >>Hypertrophic cardiomyopathy, >>Arrhythmogenic right ventricular cardiomyopathy, >>Dilated cardiomyopathy, >>Viral myocarditis, >>Fluid shear stress and atherosclerosis
Reactivity Human,Mouse,Rat,Chicken,Globefish,Bovine,Hamster,Pig,Ovine,Cat,Dog
Applications WB,IHC,IF,ELISA
ConjugationUnconjugated
Dilution rangeIF: 1:50-200 Western Blot: 1/2000 - 1/10000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from the N-terminal region of human Actin β. AA range: 1-80
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system B-cell lymphoma,Brain,Cajal-Retzius cell,Eye,Fetal brain cortex,Foreskin,Hepatocellular car
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

11 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Chicken
Globefish
Bovine
Hamster
Pig
Ovine
Cat
Dog
ABMIUM Validated™ WB
Species Dilution Notes
Human IF: 1:50-200 Western Blot: 1/2000 - 1/10000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Mouse
Rat
Chicken
Globefish
Bovine
Hamster
Pig
Ovine
Cat
Dog
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Actin β
Also known as
ACTB; Actin; cytoplasmic 1; Beta-actin; Actin β;β-Actin;Beta actin
Concentration
1 mg/ml
Working concentration
IF: 1:50-200 Western Blot: 1/2000 - 1/10000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat,Chicken,Globefish,Bovine,Hamster,Pig,Ovine,Cat,Dog
Tested applications
WBIHCIFELISA
Immunogen
Synthesized peptide derived from the N-terminal region of human Actin β. AA range: 1-80
Observed band
42kD
Cellular localization
Cytoplasm, cytoskeleton . Nucleus . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. .
Dilution range
IF: 1:50-200 Western Blot: 1/2000 - 1/10000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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