Recombinant Rat TNF-Alpha protein

Background

Tumor necrosis factor alpha (TNF-α), also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.

Expression system

Escherichia coli.

Purity

> 98 % by SDS-PAGE and HPLC analyses.

Molecular weight

Approximately 17.2 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids.

Endotoxin

Less than 1 EU/μg of rRtTNF-α as determined by LAL method.

Form

Sterile Filtered White lyophilized (freeze-dried) powder. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.2, 150 mM NaCl.

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